Cancer Gene Cloning Lab (CGCL)
Mrs S Merson
Institute of Cancer Research
High Throughput Robotics
The CGCL provides a range of microbiology and molecular genetics
techniques that are utilized by the National Prostate Cancer Collaborative.
The CGCL offers the following facilities:
- DNA Sequencing
- Allelotyping and LOH studies
- Taqman real time PCR studies
- Robotic Colony picking
- Robotic Genetic library gridding
- Robotic sample handling
- Microarraying Robots
The instruments systems in place in the lab to carry out the above
ABI Prism 3100 Genetic Analyser
The CGCL currently has two 3100s. The 3100 is a capillary
based system which automatically analyses DNA Molecules labelled
with multi-colour flourescent dyes. The 3100 is suitable for DNA
Sequencing, giving base pair reads up to 1kb which can be used for
sequence conformation of clones and mutation analysis, and fragment
analysis such as alleotyping (linkage) and loss of Heterozy Gosity
ABI Prism 377 Genetic Analyser
The CGCL has one 377. The 377 is a gel based system which automatically
analyses DNA molecules labelled with multi-colour flourescent dyes.
The 377 acts as a back up for the 3100s when they are working at
ABI Prism 7000 "TAQMAN" Real Time PCR Machine
The instruments is used by the CGCL to look fo amplifications of
specific genes within a large range of test samples.
Genomic Solutions, Flexys Robot
This robot is used to pick colonies from agar plates into 96 or
384 well culture plates. It is also used to grid genetic libraries
onto nylon filters that can be used in hybridisation techniques.