Prostate Tissue Arrays
Tissue arrays constructed by
Prof Colin Cooper firstname.lastname@example.org,
A Fletcher email@example.com,
Dr A Falconer firstname.lastname@example.org,
Dr Ros Eeles email@example.com
Institute of Cancer Research
and Prof Chris Foster, University
Tissue arrays are being constructed as part of the Collaboratives’
Molecular Pathology programme. An H&E stained section of the
formalin fixed tumour is first examined by a histopathologist who
marks the location of the tumour and normal tissue.
To make tissue arrays a block of Lambwax is first placed in the
recipient block holder in the Tissue Arrayer (Fig 1).
A core is taken of wax and is then removed (Fig 2a). A bridge is
then placed over the recipient wax block. The block containing the
formalin preserved prostate specimen together with the H&E stained
section aligned on top is then placed on the bridge (Fig 2b).
Fig 2a taking core from recipient
Fig 2b bridge and block and slide
Their position is then adjusted so that the coring needle is aligned
with the region of the tumour to be sampled. The core is then taken
(Fig 2c). Following removal of the bridge the core is then placed
in the hole in the recipient block (Figure 2d). The procedure is
repeated to construct the entire array. For each cancer six 0.6mm
cores are taken from the formalin preserved specimens two from each
of the two major areas contributing to the Gleason score and two
from regions of normal tissue. Finally, sections from the tissue
array are mounted on slides and used for immunohistochemical, DNA
and RNA analyses.
Fig 2c taking core from block
Fig 2d placing core in recipient
The NCRI Collaborative have currently constructed tissue arrays
form 100 prostate cancers. Construction of arrays from a further
100 specimens is underway. The Core Tissue Array Facility is also
being used by Professor Jack Cuzick to construct tissue arrays from
2000 prostate cancers.
The benefits of tissues arrays are numerous. They include the analysis
of multiple markers over a large number of tumours in a relatively
short space of time, generating large data sets very quickly. Tissue
arrays are very economical, instead of hundreds of experiments on
hundreds of different slides. Only a few experiments on a few slides
containing arrays would be needed, reducing costs in the reagents
and manual labour departments.
To use the Collaboratives’ prostate tissue array please email:-