National Cancer Research Institute South of England
Prostate Cancer Collaborative
Research

Development of Normal Prostate

Authors
C Butler, A Swain
Institute of Cancer Research
Source of funding: NCRI

Developmental Genetics of the Prostate
Given that carcinogenesis often involves deregulation of developmental regulatory genes, a molecular analysis of normal development of the prostate should provide important insight into prostate cancer. Although the androgen-dependent nature of prostate development has been well established, it is not clear how androgens regulate prostate differentiation and growth. Identification of the gene pathways regulated by androgens during prostate development is essential for the understanding of normal and disease processes of this male-specific organ. The use of high-density cDNA hybridisation arrays and subtractive hybridisation techniques can identify these gene pathways. These can be further studied by transgenic analysis in mouse models.
In a collaboration with Dr. Bento Soares (University of Iowa) we have developed a normalised cDNA library derived from neonatal mouse prostate. We are screening this library to identify overexpressed clones in the developing male prostate compared to female tissues. Genes of interest will be sequenced and their expression characterised by whole-mount in situ hybridisation and subjected to functional analysis using in vitro organ cultures, transgenic and/or targeted mutagenesis in the mouse. Genes identified in this project may provide useful prognostic markers for early prostate cancer.

Urogenital system of the mouse

Developing epithelial buds of the prostate - marked by the gene Nkx3.1

Expression of E-cadherin in the developing seminal vesicles


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Aetiology and Genetics

Epidemiological Identification of Families
Genetic Susceptibility
Diet and Environment
Chemoprevention

Molecular Pathology

Links to Cancer Genome Project
Development of Normal Prostate
Microarray Expression Profiling
Candidate Genes
Novel Telomerase Suppressor Genes
Subtractive Hybridization
CESH

Novel Therapies

New Drugs for Prostate Cancer
Intensity Modulated Radiotherapy
Immunotherapy
Novel Targets from Cancer Genome Project
Novel Mechanism Based Drugs

Core Resources

Cancer Gene Cloning Lab
Prostate Tissue arrays
Microarray laboratory
Tissue and blood collections
Bioinformatics

Pilot and Development Funds

Tumor micro-environment in early prostate cancer

Collaborations
Meetings and Seminars

Contact us on Email: cbell@icr.ac.uk Tel: 0208 643 8901 Fax: 0208 770 7290 This page last modified: 18/11/02