National Cancer Research Institute South of England
Prostate Cancer Collaborative

Expression profiling laser capture microdissected prostate tissues


Institute of Cancer Research


The aim of the proposed work is to obtain preliminary data of the chromosome regions and genes associated with differential expression during prostate cell differentiation and tumourigenesis.  Cells positive for markers associated with stem cell differentiation, apparently normal tissues adjacent to tumours and putative precursor lesions of prostate carcinoma, including those in different population groups with apparently different rates of progression to invasive disease, will be studied in collaborations including David Hudson and Yong-Jie Lu.  Laser capture microdissection (LCM) and associated processing of snap frozen and fresh material will be established for profiling expression changes in these cells.   Chromosomal level data will be obtained using a rapid global screening approach we have developed termed comparative expressed sequence hybridisation (CESH) and will complement microarray expression profiling of selected samples.  Although beyond the scope of the current study, genes ultimately identified may be investigated in more functional studies and assessed for their predictive value or suitability as targets for novel therapies.


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Aetiology and Genetics

Epidemiological Identification of Families
Genetic Susceptibility
Diet and Environment

Molecular Pathology

Links to Cancer Genome Project
Development of Normal Prostate
Microarray Expression Profiling
Candidate Genes
Novel Telomerase Suppressor Genes
Subtractive Hybridization

Novel Therapies

New Drugs for Prostate Cancer
Intensity Modulated Radiotherapy
Academic Urology Unit
Novel Targets from Cancer Genome Project
Novel Mechanism Based Drugs

Core Resources

Cancer Gene Cloning Lab
Prostate Tissue arrays
Microarray laboratory
Tissue and blood collections

Pilot and Development Funds

Tumor micro-environment in early prostate cancer

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Contact us on Email: Tel: 0208 643 8901 Fax: 0208 770 7290 This page last modified: 20/11/03